Basic Information
Strain Name
C57BL/6-Il12atm1(IL12A)Il12btm1(IL12B)/Bcgen
Stock Number
120552
Common Name
B-hIL12A/hIL12B mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
IL12A: interleukin 12A, P35, IL12-A; IL12B: interleukin 12B, IL-12B
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID
-
mRNA expression analysis
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RT-PCR analysis of IL12A and IL12B gene.
The human IL12A but not mouse Il12a mRNA was detectable in splenocyte of the homozygous B-hIL12A/hIL12B mice.
The human IL12B but not mouse Il12b mRNA was detectable in thymus cell of the homozygous B-hIL12A/hIL12B mice.
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Protein expression analysis
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Strain specific IL12(heterodimer p70) expression analysis in homozygous B-hIL12A/hIL12B mice by ELISA.
Serum was collected om WT (+/+) and homozygous B-hIL12A/IL12B mice (H/H) mice stimulated with anti-mCD3ε and anti-mCD28 in vivo (n=3), and analyzed by ELISA with species-specific IL12 ELISA kit. Mouse IL12 (p70) was detectable in WT mice. Human IL12 (p70) was exclusively detectable in homozygous B-hIL12A/IL12B mice (H/H) mice but not in WT mice. ND: Not detectable.
