Protein expression analysis
Strain specific IL12(heterodimer p70) expression analysis in homozygous B-hIL12A/hIL12B mice by ELISA. Serum was collected from WT (+/+) and homozygous B-hIL12A/IL12B mice (H/H) mice stimulated with LPS in vivo (n=3), and analyzed by ELISA with species-specific IL12 ELISA kit. Mouse IL12 (p70) was detectable in WT mice. Human IL12 (p70) was exclusively detectable in homozygous B-hIL12A/IL12B mice (H/H) mice but not in WT mice. ND: Not detectable.