Basic Information

Strain Name
C57BL/6-Itgb7tm1(ITGB7)/Bcgen
Common Name
B-hITGB7 mice
Background
C57BL/6
Catalog Number
111246
NCBI Gene ID
Related Gene
integrin subunit beta 7, Ly69

Targeting strategy

Gene targeting strategy for B-hITGB7 mice.

The exons 2-14 of mouse Itgb7 gene that encode the extracellular domain were replaced by human ITGB7 exons 3-15 in B-hITGB7 mice.

Protein expression analysis

Strain specific ITGB7 expression analysis in heterozygous B-ITGB7 mice by flow cytometry.

Splenocytes were collected from wild-type C57BL/6 mice and heterozygous B-hITGB7 mice (H/+), and analyzed by flow cytometry with species-specific anti-ITGB7 antibody. Mouse ITGB7 was detectable in wild-type mice and heterozygous B-hITGB7 mice (H/+). Human ITGB7 was exclusively detectable in heterozygous B-ITGB7 mice but not in wild-type mice.

Strain specific ITGB7 expression analysis in heterozygous B-ITGB7 mice by flow cytometry.

Splenocytes were collected from wild-type C57BL/6 mice and heterozygous B-hITGB7 mice (H/+), and analyzed by flow cytometry with species-specific anti-ITGB7 antibody. Mouse ITGB7 was detectable in wild-type mice and heterozygous B-hITGB7 mice (H/+). Human ITGB7 was exclusively detectable in heterozygous B-ITGB7 mice but not in wild-type mice.

Summary

Protein expression

Mouse ITGB7 was detectable in wild-type mice and heterozygous B-hITGB7 mice (H/+). Human ITGB7 was exclusively detectable in heterozygous B-ITGB7 mice but not in wild-type mice.