Basic Information
Strain Name
C57BL/6N-Gt(ROSA)26Sortm1(KAAG1)Bcgen/Bcgen
Common Name
B-hKAAG1 mice
Catalog Number
112188
Background
C57BL/6N
Related Gene
KAAG1: RU2AS
NCBI Gene ID
-
mRNA expression analysis
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Strain specific analysis of KAAG1 mRNA expression in wild-type C57BL/6N mice and B-hKAAG1 mice by RT-PCR. Kidney and testis RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hKAAG1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with two human KAAG1 primers. As control, a group without reverse enzyme was also set. Human KAAG1 mRNA was detectable in homozygous B-hKAAG1 mice but not in wild-type mice and was confirmed via Sanger sequencing.