Basic Information

Strain Name
C57BL/6-B2mtm2(B2M/HLA-A2.1/H2-D)/Bcgen
Stock Number
110110
Common Name
B-HLA-A2.1 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
HLA
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

Transgenic mice that express human HLA molecules represent a unique in vivo experimental model for evaluating human immune system function.These models have been used to study the role of the human class I or class Il restricted T cell repertoire in autoimmune disease, infectious disease, and vaccine development. They are also valued tools for evaluating human HLA restrictec T cell-mediated vaccine efficacy in oncology applications. Biocytogen developed the B-HLA 2.1 mice. The model B2m gene (Exon1 to Exon3) of mouse were replaced by the sequence encompassing the human B2M CDS and HLA-A*0201 gene that included leader sequeTargetednce, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains. This mice express human HLA molecules have helped advance the science related to human immune system function, as well as potential novel therapeutics.

Targeted

Targeted allele

Gene targeting strategy for B-HLA-2.1 mice. The B2M gene (Exon1 to Exon3) of mouse were replaced by the sequence encompassing the human B2M CDS and HLA-A*0201 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains.

Details

Protein expression analysis ((homozygous mice))

Strain specific B2M and HLA expression analysis in homozygous B-HLA-A2.1 mice by flow cytometry. Splenocytes from both wild type (+/+) C57BL/6 and homozygous B-HLA-A2.1 (H/H) mice were analyzed by flow cytometry. Mouse B2M and H-2K/H-2D were detectable in the WT C57BL/6. Human B2M and HLA-A2 were only detectable in the homozygous B-HLA-A2.1 mice.

 

Protein expression analysis (homozygous mice)

Strain specific B2M and HLA expression analysis in homozygous B-HLA-A2.1 mice by flow cytometry. Splenocytes from both wild type (+/+) C57BL/6 and homozygous B-HLA-A2.1 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry. Mouse B2M and H-2K/H-2D were detectable in the WT C57BL/6. Human B2M and HLA-A2 were only detectable in the homozygous B-HLA-A2.1 mice.

Analysis of spleen leukocytes cell subpopulations in B-HLA-A2.1 mice

Analysis of spleen leukocyte subpopulations by FACS Splenocytes were isolated from C57BL/6 and B-HLA-A2.1 mice.The proportion of lymphocyte subpopulation was tested by flow cytometry. Single live cells were gated for CD45 population and used for further analysis as indicated here. As a result, the expression profile of lymphocyte subpopulation in homozygous B-HLA-A2.1 mice is similar to that in the C57BL/6 mice, indicating that differentiation of T cell and B cell are not affected by the humanization of HLA-A2.1. While the peripheral CD8+ T cell population in B-HLA-A2.1 mice was lower than C57BL/6 (exception the second mice ).

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