Basic Information
Strain name
C57BL/6-Igs2tm1(MICA)Bcgen/Bcgen
Common name
B-hMICA mice
Background
C57BL/6
Catalog number
112161
Aliases
MIC-A, PERB11.1
NCBI gene ID
-
Targeting strategy
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Gene targeting strategy for B-hMICA mice.
The human gene coding sequence was inserted to mouse Hipp11(H11) allele in B-hMICA mice.
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Protein expression analysis in spleen T, B and NK cells
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Strain specific MICA expression analysis in wild type C57BL/6 mice and homozygous humanized B-hMICA mice by flow cytometry. Splenocytes were collected from wild type C57BL/6 mice (+/+) and homozygous B-hMICA mice (H/H), stimulated with PMA+ionomycin cocktail in vitro ( Cells were cultured in presence of PMA/I for 2 h, extensively washed, and subsequently cultured for up to 22 h) and analyzed by flow cytometry with species-specific MICA/MICB antibody. Human MICA was exclusively detectable on T cells, B cells and NK cells of homozygous B-hMICA mice.
