Basic Information
Strain Name
C57BL/6-Igs2tm1(NCR2)/Bcgen
Common Name
B-hNCR2 mice
Background
C57BL/6
Catalog Number
111872
NCBI Gene ID
Related Genes
LY95; CD336; NKP44; NK-p44; dJ149M18.1
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Targeting strategy
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Gene targeting strategy for B-hNCR2 mice. The human gene coding sequence was inserted to mouse Hipp11(H11) allele in B-hNCR2 mice.
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Protein expression analysis
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Strain specific NCR2 expression analysis in homozygous B-hNCR2 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hNCR2 mice (H/H), and then were cultured for 4 days under 50ng/mL hIL12 and hIL15 stimulation ex vivo. Flow cytometry was performed with anti-human NCR2 antibody to detect NCR2 expression. Human NCR2 was exclusively detectable in NK cells of homozygous B-hNCR2 mice. -
Summary
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Protein expression analysis:
Human NCR2 was exclusively detectable in homozygous B-hNCR2 mice but not wild-type mice.
