Basic Information
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Targeting strategy
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Gene targeting strategy for B-hNKG2D mice plus. The 5’UTR and exons 2~8 of mouse Nkg2d gene encoding the full-length were replaced by 5’UTR and exons 2~8 of human NKG2D in B-hNKG2D mice plus.
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Protein expression analysis in CD4+T cells
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Strain specific analysis of NKG2D expression in wild-type (WT) mice (+/+) and homozygous B-hNKG2D mice plus(H/H) by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNKG2D mice plus (H/H), and analyzed by flow cytometry with species-specific anti-NKG2D antibody. mNKG2D and hNKG2D were not detectable in CD4+T cells of wild-type and homozygous B-hNKG2D mice plus.
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Protein expression analysis in CD8+T cells
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Strain specific analysis of NKG2D expression in wild-type (WT) mice (+/+) and homozygous B-hNKG2D mice plus(H/H) by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNKG2D mice plus(H/H), and analyzed by flow cytometry with species-specific anti-NKG2D antibody. mNKG2D was not detectable in CD8+T cells of wild-type C57BL/6 mice, while hNKG2D was detectable in homozygous B-hNKG2D mice plus.
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Protein expression analysis in NK cells
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Strain specific analysis of NKG2D expression in wild-type (WT) mice (+/+) and homozygous B-hNKG2D mice plus(H/H) by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNKG2D mice plus(H/H), and analyzed by flow cytometry with species-specific anti-NKG2D antibody. mNKG2D was detectable in NK cells of wild-type C57BL/6 mice and homozygous B-hNKG2D mice plus, while hNKG2D was only detectable in homozygous B-hNKG2D mice plus.
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Summary
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Protein expression analysis:
Human NKG2D was exclusively detectable in homozygous B-hNKG2D mice plus but not wild-type mice, and mouse NKG2D was detectable in wild-type mice.
