Protein Expression Analysis
Splenocytes from homozygous B-hPD-1/hCD40 mice were analyzed by flow cytometry. Mouse CD40+ and PD-1+ T cells were detectable in wild type (WT) C57BL/6, while hCD40+ and hPD-1+ T cells were detectable in the homozygous B-hPD-1/hCD40 mice.
Antibody Efficacy Evaluation
Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/hCD40 mice. Mice were grouped when the tumor size was approximately 150±50 mm3 (n=8). Anti-hCD40 antibody Selicrelumab Analog and the anti-hPD-1 antibody Keytruda show modest inhibition of tumor growth. On the other hand, combination of Selicrelumab Analog and Keytruda shows significantly inhibition when compared to single Ab treatment. B-hPD-1/hCD40 mouse model is a powerful tool for in vivo evaluation of combination therapy efficacy for hPD-1 antibodies and hCD40 antibodies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.
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