B-hPD-1/hOX40 mice

Basic Information

Targeting Strategy

Details

Phenotype

mRNA Expression Analysis

RT-PCR analysis of the B-hPD-1/hOX40 humanized mice.

The splenocytes of the B-hPD-1/hOX40 homozygous mice showed expression of human PD-1 and OX40 mRNA but not mouse PD-1 and OX40 mRNA.

Protein Expression Analysis

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hOX40 mice were analyzed by flow cytometry. Mouse OX40+ T cells were detectable in the WT C57BL/6 mice, while human OX40+ T cells were detectable in the homozygous B-hPD-1/hOX40 mice.

 

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hOX40 mice were analyzed by flow cytometry. Mouse PD-1+ T cells were detectable in the WT C57BL/6 mice, while human PD-1+ T cells were detectable in the homozygous B-hPD-1/hOX40 mice.

Application

Combination Therapy of PD-1(Keytruda) Ab and OX40 Ab

Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/hOX40 mice. Mice were grouped when the tumor size was approximately 150±50 mm³ (n=6).  Anti-hOX40 antibody and the anti-hPD-1 antibody Keytruda show inhibitory effects on tumor growth. On the other hand, combination of anti-hOX40 antibody and the anti-hPD-1 antibody Keytruda shows almost complete inhibition when compared to single Ab treatment. B-hPD-1/hOX40 mouse model is a powerful tool for is a powerful tool for in vivo efficacy evaluation of hPD-1 and hOX40 antibodies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

Combination Therapy of PD-1(Keytruda) Ab and OX40 Ab

Murine melanoma B16-F10-hPD-L1 cells were subcutaneously implanted into homozygous B-hPD-1/hOX40 mice.Mice were grouped when the tumor size was approximately 150±50 mm3 (n=5). Combination of anti-hOX40 antibody and the anti-hPD-1 antibody Keytruda shows higher inhibitory effects than single antibody treatment. B-hPD-1/hOX40 mouse model is a powerful tool for is a powerful tool for in vivo efficacy evaluation of hPD-1 and hOX40 antibodies combination therapy. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

References

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