Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPD-1 plus/hIL21R mice. Human PD-1 gene encoding the extracellular region and mouse PD-1 gene encoding the transmembrane and cytoplasmic region were inserted after the initiation codon ATG of mouse PD-1 gene in B-hPD-1 plus/hIL21R mice. The exons 3-6 of mouse Il21r gene that encode the extracellular domain were replaced by human IL21R exons 3-6 in B-hPD-1 plus/hIL21R mice.
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Protein m/hIL21R expression analysis in spleen
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Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with species-specific IL21R antibody. Human IL21R was weakly detectable in homozygous B-hPD-1 plus/hIL21R mice.
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Protein m/hPD-1 expression analysis in spleen
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Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with species-specific PD-1 antibody. Human PD-1 was exclusively detectable in homozygous B-hPD-1 plus/hIL21R mice.
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Summary
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- Protein expression analysis:
Human IL21R and PD-1 were detectable in homozygous B-hPD-1 plus/hIL21R mice.
