Basic Information

Strain Name
C57BL/6-Pdcd1tm3(PDCD1)Il21rtm1(IL21R)/Bcgen
Common Name
B-hPD-1 plus/hIL21R mice
Background
C57BL/6
Catalog number
111847
Aliases
CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1;CD360, IMD56, NILR
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hPD-1 plus/hIL21R mice. Human PD-1 gene encoding the extracellular region and mouse PD-1 gene encoding the transmembrane and cytoplasmic region were inserted after the initiation codon ATG of mouse PD-1 gene in B-hPD-1 plus/hIL21R mice. The exons 3-6 of mouse Il21r gene that encode the extracellular domain were replaced by human IL21R exons 3-6 in B-hPD-1 plus/hIL21R mice.

Protein m/hIL21R expression analysis in spleen

Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with species-specific IL21R antibody. Human IL21R was weakly detectable in homozygous B-hPD-1 plus/hIL21R mice.

Protein m/hPD-1 expression analysis in spleen

Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with species-specific PD-1 antibody. Human PD-1 was exclusively detectable in homozygous B-hPD-1 plus/hIL21R mice.

Summary

  • Protein expression analysis:

Human IL21R and PD-1 were detectable in homozygous B-hPD-1 plus/hIL21R mice.

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