Basic Information
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Protein m/hIL21R expression analysis in spleen
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Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with species-specific IL21R antibody. Human IL21R was weakly detectable in homozygous B-hPD-1 plus/hIL21R mice.
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Protein m/hPD-1 expression analysis in spleen
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Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with species-specific PD-1 antibody. Human PD-1 was exclusively detectable in homozygous B-hPD-1 plus/hIL21R mice.
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Protein m/hPD-1 expression analysis in spleen
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Strain specific PD-1 expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with species-specific PD-1 antibody. Mouse PD-1 was detectable in wild-type mice. Human PD-1 was exclusively detectable in homozygous B-hPD-1 plus/hIL21R mice.
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Protein m/hIL21R expression analysis in spleen
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Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with anti-hIL21R antibody. Human IL21R was detectable in T, B and NK cells of homozygous B-hPD-1 plus/hIL21R mice. The anti-hIL21R antibody is crossly reactive with IL21R in human and mice.
Strain specific IL21R expression analysis in B-hPD-1 plus/hIL21R mice by flow cytometry. Splenocytes were collected from C57BL/6 (+/+) and homozygous B-hPD-1 plus/hIL21R mice (H/H), and analyzed by flow cytometry with anti-hIL21R antibody. Human IL21R was detectable in T, B and NK cells of homozygous B-hPD-1 plus/hIL21R mice. The anti-hIL21R antibody is crossly reactive with IL21R in human and mice.
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Analysis of leukocytes cell subpopulation in spleen
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Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hPD-1 plus/hIL21R mice(n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hPD-1 plus/hIL21R were similar to those in the C57BL/6 mice, demonstrating that introduction of hPD-1 and hIL21R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
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Analysis of T cell subpopulation in spleen
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Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hPD-1 plus/hIL21R mice(n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hPD-1 plus/hIL21R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPD-1 and hIL21R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.
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Analysis of leukocytes cell subpopulation in blood
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Analysis of blood leukocyte subpopulations by FACS. Blood were isolated from female C57BL/6 and homozygous B-hPD-1 plus/hIL21R mice(n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hPD-1 plus/hIL21R were similar to those in the C57BL/6 mice, demonstrating that introduction of hPD-1 and hIL21R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
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Analysis of T cell subpopulation in blood
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Analysis of blood T cell subpopulations by FACS. Blood were isolated from female C57BL/6 and homozygous B-hPD-1 plus/hIL21R mice(n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hPD-1 plus/hIL21R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPD-1 and hIL21R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in blood. Values are expressed as mean ± SEM.
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Analysis of leukocytes cell subpopulation in lymph nodes
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Analysis of lymph nodes leukocyte subpopulations by FACS. Lymph nodes were isolated from female C57BL/6 and homozygous B-hPD-1 plus/hIL21R mice(n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hPD-1 plus/hIL21R were similar to those in the C57BL/6 mice, demonstrating that introduction of hPD-1 and hIL21R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph nodes. Values are expressed as mean ± SEM.
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Analysis of T cell subpopulation in lymph nodes
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Analysis of lymph nodes T cell subpopulations by FACS. Lymph nodes were isolated from female C57BL/6 and homozygous B-hPD-1 plus/hIL21R mice(n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hPD-1 plus/hIL21R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hPD-1 and hIL21R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in lymph nodes. Values are expressed as mean ± SEM.
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Function analysis
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Mouse pSTAT3 was induced with mouse IL21 and human IL21 in homozygous B-hPD-1 plus/hIL21R mice analyzed by flow cytometry. Splenocytes were collected from homozygous B-hPD-1 plus/hIL21R mice (H/H), and stimulated with culture medium, mIL21 or hIL21. The induction of STAT5 phosphorylation on B cells with the indicated stimulators was assayed by flow cytometry. Results indicated that STAT5 phosphorylation was successfully induced with mouse IL21 or human IL21 in homozygous B-hPD-1 plus/hIL21R mice.
Mouse pSTAT3 was induced with mouse IL21 and human IL21 in homozygous B-hPD-1 plus/hIL21R mice analyzed by flow cytometry. Splenocytes were collected from homozygous B-hPD-1 plus/hIL21R mice (H/H), and stimulated with culture medium, mIL21 or hIL21. The induction of STAT5 phosphorylation on CD8+T cells with the indicated stimulators was assayed by flow cytometry. Results indicated that STAT5 phosphorylation was successfully induced with mouse IL21 or human IL21 in homozygous B-hPD-1 plus/hIL21R mice.
Mouse pSTAT3 was induced with mouse IL21 and human IL21 in homozygous B-hPD-1 plus/hIL21R mice analyzed by flow cytometry. Splenocytes were collected from homozygous B-hPD-1 plus/hIL21R mice (H/H), and stimulated with culture medium, mIL21 or hIL21. The induction of STAT5 phosphorylation on CD4+T cells with the indicated stimulators was assayed by flow cytometry. Results indicated that STAT5 phosphorylation was successfully induced with mouse IL21 or human IL21 in homozygous B-hPD-1 plus/hIL21R mice.
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Summary
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- Protein expression analysis:
Human IL21R and PD-1 were detectable in homozygous B-hPD-1 plus/hIL21R mice.
