Basic Information
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Targeting strategy
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Gene targeting strategy for B-hRAGE mice plus.
Exons 1-11 of the mouse RAGE gene that encodes the full-length protein was replaced by human RAGE exons 1-11 in B-hRAGE mice plus.
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mRNA expression analysis
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Strain specific analysis of RAGE mRNA expression in wild-type C57BL/6 mice and B-hRAGE mice plus by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hRAGE mice plus (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human RAGE primers. Mouse RAGE mRNA was detectable only in wild-type C57BL/6 mice. Human RAGE mRNA was detectable only in homozygous B-hRAGE mice plus but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of RAGE protein expression in homozygous B-hRAGE mice plus. Lung tissue lysates were collected from wild-type C57BL/6N mice (+/+), homozygous B-hRAGE mice plus (H/H) and B-Ager KO mice(RM), and then analyzed by western blot with species-specific anti-RAGE antibody. 40 μg total proteins were loaded for western blotting analysis. RAGE was detected in lung.
