Basic Information

Strain Name
C57BL/6-Sirpatm1(SIRPA)Cd47tm1(CD47)Il2rgtm1/Bcgen
Stock Number
130566
Common Name
B-hSIRPA/hCD47,Il2rg KO mice
Background
C57BL/6
Related Genes
BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP, IAP, MER6, OA3,  IL-2RG, IMD4, P64, SCIDX, SCIDX1

Protein expression analysis

Species specific SIRPα expression analysis in B-hSIRPA/hCD47,Il2rg KO mice by flow cytometry. Splenocytes isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Il2rg KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-SIRPα antibodies. Human SIRPα was exclusively detectable in homozygous B-hSIRPA/hCD47,Il2rg KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 and homozygous B-hSIRPA/hCD47,Il2rg KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.

Species specific CD47 expression analysis in B-hSIRPA/hCD47,Il2rg KO mice by flow cytometry. Splenocytes were isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Il2rg KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-CD47 antibodies. Mouse CD47 was only detectable in C57BL/6 mice. Human CD47 was exclusively detectable in homozygous B-hSIRPA/hCD47,Il2rg KO mice but not in C57BL/6 mice. Mouse and human CD47 were not detectable on T cells when stimulated with anti-mCD3ε antibody.