Basic Information

Strain Name
C57BL/6-Sirpatm1(SIRPA)Cd47tm1(CD47)Prkdctm1/Bcgen
Stock Number
130565
Common Name
B-hSIRPA/hCD47, Prkdc KO mice
Background
C57BL/6
NCBI Gene ID
SIRPA: 140885; CD47: 961; Prkdc: 19090
Related Genes
BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP, IAP, MER6, OA3, DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350, CD132, CIDX

Protein expression analysis

Species specific SIRPα expression analysis in B-hSIRPA/hCD47,Prkdc KO mice by flow cytometry. Splenocytes isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-SIRPα antibodies. Human SIRPα was exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 and homozygous B-hSIRPA/hCD47,Prkdc KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.

Species specific CD47 expression analysis in B-hSIRPA/hCD47,Prkdc KO mice by flow cytometry. Splenocytes were isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-CD47 antibodies. Mouse CD47 was only detectable in C57BL/6 mice. Human CD47 was exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc KO mice but not in C57BL/6 mice. Human CD47 was not detectable on T cells in B-hSIRPA/hCD47,Prkdc KO mice stimulated with or without anti-mCD3ε antibody.