Basic Information
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Protein expression analysis
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Species specific SIRPα expression analysis in B-hSIRPA/hCD47,Prkdc KO mice by flow cytometry. Splenocytes isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-SIRPα antibodies. Human SIRPα was exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 and homozygous B-hSIRPA/hCD47,Prkdc KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.
Species specific CD47 expression analysis in B-hSIRPA/hCD47,Prkdc KO mice by flow cytometry. Splenocytes were isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc KO mice (H/H) stimulated with or without anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-CD47 antibodies. Mouse CD47 was only detectable in C57BL/6 mice. Human CD47 was exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc KO mice but not in C57BL/6 mice. Human CD47 was not detectable on T cells in B-hSIRPA/hCD47,Prkdc KO mice stimulated with or without anti-mCD3ε antibody.
