Basic Information

Strain Name
C57BL/6-Tgfb1tm2(TGFB1)/Bcgen
Common Name
B-hTGFB1 mice
Background
C57BL/6
Catalog number
112245
Aliases
TGFB1(CED, LAP, DPD1, TGFB, IBDIMDE, TGFbeta, TGF-beta1)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTGFB1 mice.

The human TGFB1 gene (encoding the full-length protein) was inserted following part of exon 2 of the mouse Tgfb1 gene to generate B-hTGFB1 mice.

mRNA expression analysis

Strain specific analysis of TNFR2 gene expression in wild-type C57BL/6 mice and hTGFB1 mice by RT-qPCR. mRNA expression of hTGFB1 in homozygous B-hTGFB1 mice (H/H) was similar to those in wild-type C57BL/6 mice (+/+), demonstrating that introduction of hTGFB1 in place of its mouse counterpart does not change the expression level of TGFB1 mRNA.

Protein expression analysis

Strain specific TGFB1 expression analysis in homozygous B-hTGFB1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTGFB1 mice (H/H), and analyzed by flow cytometry with species-specific anti-TGFB1 antibody. Mouse TGFB1 was detectable in wild-type mice. Human TGFB1was exclusively detectable in homozygous B-hTGFB1 but not in wild-type mice.

Analysis of leukocytes in spleen

Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous humanized B-hTGFB1 mice(n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hTGFB1 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTGFB1 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.

Analysis of T cell subpopulations in spleen

Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous humanized B-hTGFB1 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hTGFB1 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTGFB1 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.

Analysis of leukocytes in lymph node

Analysis of lymph node leukocyte subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and homozygous humanized B-hTGFB1 mice(n=3, 6-week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of T cells, B cells and NK cells in homozygous B-hTGFB1 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTGFB1 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in lymph node

Analysis of lymph node T cell subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and homozygous B-hTGFB1 mice(n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hTGFB1 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTGFB1 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in lymph node. Values are expressed as mean ± SEM.

Analysis of leukocytes in blood

Analysis of blood leukocyte subpopulations by FACS. Blood were isolated from female C57BL/6 and homozygous B-hTGFB1 mice(n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hTGFB1 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTGFB1 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Analysis of T cell subpopulations in blood

Analysis of blood T cell subpopulations by FACS. Blood were isolated from female C57BL/6 and homozygous B-hTGFB1 mice(n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hTGFB1 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTGFB1 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in blood. Values are expressed as mean ± SEM.

Platelet Expression

Analysis of platelets in B-hTGFB1 mice by FACS. Blood was isolated from female humanized B-hTGFB1 mice. Flow cytometry analysis of the platelets was performed to assess human LAP expression. LAP was detectable on wild-type C57BL/6 mice and homozygous B-hTGFB1 mice.