Basic Information
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Gene Targeting Strategy
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The chimeric coding sequence (mouse signal peptide, human extracellular domain and mouse transmembrane and intracellular domain) was inserted to replace part of murine Tgfbr2 exon 2 B-hTGFBR2 mice.
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Protein Expression Analysis
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Species-specific TGFBR2 expression analysis in humanized B-hTGFBR2 mice. Splenocytes were collected from wild-type C57BL/6 (+/+) and heterozygous B-hTGFBR2 (H/+) mice and analyzed by flow cytometry using species-specific anti-TGFBR2 antibodies. Murine TGFBR2 was detected in wild-type and heterozygous B-hTGFBR2 mice, while human TGFBR2 was exclusively detected in B-hTGFBR2 mice.
