TNFSF15, TL1, TL1A, TNLG1B, VEGI, VEGI192A, TNF superfamily member 15
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hTL1 mice. Exons 1-4 of mouse Tl1 gene that encode the extracellular domain were replaced by human TL1 exons 1-4 in B-hTL1 mice.
mRNA expression analysis
Species-specific TL1 gene expression analysis in wild-type and humanized B-hTL1 mice by RT-PCR. Murine Tl1 mRNA was detected in lung tissue isolated from wild-type (+/+) mice, while human TL1 mRNA was detected in homozygous B-hTL1 (H/H) mice.
Protein expression analysis
Species-specific TL1 protein expression analysis in wild-type and humanized B-hTL1 mice. Lung endothelial cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hTL1 (H/H) mice and analyzed by flow cytometry using a cross-reactive anti-m/hTL1 antibody. TL1 protein was detected in wild-type and B-hTL1 mice.