Basic Information
Strain Name
C57BL/6-Tnfsf15tm2(TNFSF15)/Bcgen
Common Name
B-hTL1 mice
Background
C57BL/6N
Catalog number
111997
Aliases
TNFSF15, TL1, TL1A, TNLG1B, VEGI, VEGI192A, TNF superfamily member 15
NCBI Gene ID
-
Gene targeting strategy
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Gene targeting strategy for B-hTL1 mice. Exons 1-4 of mouse Tl1 gene that encode the extracellular domain were replaced by human TL1 exons 1-4 in B-hTL1 mice.
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mRNA expression analysis
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Species-specific TL1 gene expression analysis in wild-type and humanized B-hTL1 mice by RT-PCR. Murine Tl1 mRNA was detected in lung tissue isolated from wild-type (+/+) mice, while human TL1 mRNA was detected in homozygous B-hTL1 (H/H) mice.
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Protein expression analysis
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Species-specific TL1 protein expression analysis in wild-type and humanized B-hTL1 mice. Lung endothelial cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hTL1 (H/H) mice and analyzed by flow cytometry using a cross-reactive anti-m/hTL1 antibody. TL1 protein was detected in wild-type and B-hTL1 mice.
