Basic Information

Strain Name
BALB/c-Tnfrsf1btm1(TNFRSF1B)Bcgen/Bcgen
Common Name
B-hTNFR2 mice(C)
Background
BALB/cCrSlcNifdc
Catalog Number
112644
NCBI Gene ID
Related Genes
TNFRSF1B (Tumor necrosis factor receptor superfamily, member 1b)

Application

This product is used for pharmacodynamics and safety evaluation of cancer.

Targeting strategy

Gene targeting strategy for B-hTFNR2 mice(C). 

The exons 2-6 of mouse Tfnr2 gene that encodes the full-length protein was replaced by human TFNR2 exons 2-6 in B-hTFNR2 mice(C).

Protein expression analysis in spleen T and Treg cells

Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Human TNFR2 was exclusively detectable on T and Treg cells of homozygous B-hTNFR2 mice(C).

In vivo efficacy of anti-human TNFR2 antibody

Antitumor activity of anti-human TNFR2 antibody in B-hTNFR2 mice(C). (A) Anti-human TNFR2 antibody inhibited CT26.WT tumor growth in B-hTNFR2 mice(C). Murine colon cancer CT26.WT cells were subcutaneously implanted into homozygous B-hTNFR2 mice(C) (female, 7-week-old, n=6). Mice were grouped when tumor volume reached approximately 40-60 mm3, at which time they were intraperitoneally injected with anti-human TNFR2 antibody indicated in panel. (B) Body weight changes during treatment. As shown in panel A, anti-human TNFR2 antibody was efficacious in controlling tumor growth in B-hTNFR2 mice(C), demonstrating that the B-hTNFR2 mice(C) provide a powerful preclinical model for in vivo evaluation of anti-human TNFR2 antibodies. Values are expressed as mean ± SEM. (The hTNFR2 Ab1 was provided by the client)

Summary

Protein expression analysis:

Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C) but not wild-type mice, and mouse TNFR2 was detectable in wild-type mice. 

In vivo efficacy:

Anti-human TNFR2 antibody was efficacious in controlling tumor growth in B-hTNFR2 mice(C), demonstrating that the B-hTNFR2 mice(C) provide a powerful preclinical model for in vivo evaluation of anti-human TNFR2 antibodies.

Back to top