Basic Information
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Targeting strategy
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Gene targeting strategy for B-hTFNR2 mice(C).
The exons 2-6 of mouse Tfnr2 gene that encodes the full-length protein was replaced by human TFNR2 exons 2-6 in B-hTFNR2 mice(C). -
Protein expression analysis in T cells
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Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hTNFR2 mice(C) (H/H) stimulated with anti-mCD3ɛ antibody (7.5μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific TNFR2 antibody. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C).
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Protein expression analysis in Tregs
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Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hTNFR2 mice(C) (H/H) stimulated with anti-mCD3ɛ antibody (7.5μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific TNFR2 antibody. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C).
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Summary
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Protein expression analysis:
Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C) but not wild-type mice, and mouse TNFR2 was detectable in wild-type mice.
