Basic Information
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Application
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This product is used for pharmacodynamics and safety evaluation of cancer.
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Targeting strategy
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Gene targeting strategy for B-hTFNR2 mice(C).
The exons 2-6 of mouse Tfnr2 gene that encodes the full-length protein was replaced by human TFNR2 exons 2-6 in B-hTFNR2 mice(C).
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Protein expression analysis in spleen T and Treg cells
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Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Human TNFR2 was exclusively detectable on T and Treg cells of homozygous B-hTNFR2 mice(C).
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In vivo efficacy of anti-human TNFR2 antibody
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Antitumor activity of anti-human TNFR2 antibody in B-hTNFR2 mice(C). (A) Anti-human TNFR2 antibody inhibited CT26.WT tumor growth in B-hTNFR2 mice(C). Murine colon cancer CT26.WT cells were subcutaneously implanted into homozygous B-hTNFR2 mice(C) (female, 7-week-old, n=6). Mice were grouped when tumor volume reached approximately 40-60 mm3, at which time they were intraperitoneally injected with anti-human TNFR2 antibody indicated in panel. (B) Body weight changes during treatment. As shown in panel A, anti-human TNFR2 antibody was efficacious in controlling tumor growth in B-hTNFR2 mice(C), demonstrating that the B-hTNFR2 mice(C) provide a powerful preclinical model for in vivo evaluation of anti-human TNFR2 antibodies. Values are expressed as mean ± SEM. (The hTNFR2 Ab1 was provided by the client)
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Summary
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Protein expression analysis:
Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C) but not wild-type mice, and mouse TNFR2 was detectable in wild-type mice.
In vivo efficacy:
Anti-human TNFR2 antibody was efficacious in controlling tumor growth in B-hTNFR2 mice(C), demonstrating that the B-hTNFR2 mice(C) provide a powerful preclinical model for in vivo evaluation of anti-human TNFR2 antibodies.
