Basic Information

Strain Name
C57BL/6-Tnfsf12tm1(TNFSF12)Tnfsf13tm1(TNFSF13)/Bcgen
Common Name
B-hTWEAK/hAPRIL mice
Catalog number
110805
Background
C57BL/6J
Alias
TNFSF12; APO3L; DR3LG; TNLG4A TNFSF13; CD256; TALL2; ZTNF2; TALL-2; TNLG7B; TRDL-1; UNQ383/PRO715
NCBI Gene ID

Targeting strategy

The full sequence of mouse Tweak gene and mouse April gene were replaced with the corresponding human sequences in B-hTWEAK/hAPRIL mice.

Protein expression analysis

Strain specific TWEAK expression analysis in homozygous B-hTWEAK/hAPRIL mice by western blot.

Lung was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTWEAK/hAPRIL mice (H/H), and analyzed by western blot with anti-TWEAK antibody, which has cross reaction between human TWEAK and mouseTWEAK. TWEAK was detectable both in wild-type mice and homozygous B-hTWEAK/hAPRIL mice.

Strain specific APRIL expression analysis in heterozygous B-hTWEAK/hAPRIL mice by flow cytometry.

Splenocytes were collected from wild-type C57BL/6 mice (+/+)  and homozygous B-hTWEAK/hAPRIL mice (H/H), and analyzed by flow cytometry with anti-APRIL antibody, which has cross reaction between human APRIL and mouse APRIL. APRIL was detectable both in wild-type mice and homozygous B-hTWEAK/hAPRIL mice.