Basic Information
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Targeting Strategy
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Gene targeting strategy for B-Tg(hSIGLEC7/hSIGLEC9) mice. A BAC containing the entire human SIGLEC7 and SIGLEC9 sequence was randomly inserted into the mouse genome in B-Tg(hSIGLEC7/hSIGLEC9) mice.
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Protein Expression Analysis
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Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived NK cells were collected from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7-hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.
Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived monocytes were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.
Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived macrophages were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.
Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived granulocytes were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.
Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived T cells were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.
