Basic Information

Strain name
C57BL/6-Tg(CH17-289L2/CH17-302I14)1/Bcgen
Common name
B-Tg(hSIGLEC7/hSIGLEC9) mice
Background
C57BL/6
Catalog number
121189
Aliases
SIGLEC7 (p75; QA79; AIRM1; CD328; AIRM-1; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1) SIGLEC9 (CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE)

Targeting strategy

Gene targeting strategy for B-Tg(hSIGLEC7/hSIGLEC9) mice. The BAC containing whole human SIGLEC7 and SIGLEC9 genome sequence was inserted into mouse genome randomly in B-Tg(hSIGLEC7/hSIGLEC9) mice.

Protein expression analysis

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7-hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice mice (+/+) and B-Tg(hSiglec7-hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7-hSiglec9) mice in NK cells.

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7-hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice mice (+/+) and B-Tg(hSiglec7-hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7-hSiglec9) mice in monocytes.

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7-hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice mice (+/+) and B-Tg(hSiglec7-hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7-hSiglec9) mice in macrophages.

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7-hSiglec9) mice by flow cytometry. Blood was collected from wild-typeC57BL/6 mice  mice (+/+) and B-Tg(hSiglec7-hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7-hSiglec9) mice in granulocytes.

Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7-hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice mice (+/+) and B-Tg(hSiglec7-hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7-hSiglec9) mice in T cells.

Summary

Protein expression analysis:

Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7-hSiglec9) mice on NK cells, monocytes, macrophages, granulocytes and T cells.