Basic Information

Strain name
C57BL/6-Tg(CH17-289L2/CH17-302I14)1/Bcgen
Common name
B-Tg(hSIGLEC7/hSIGLEC9) mice
Background
C57BL/6
Catalog number
121189
Aliases
SIGLEC7 (p75; QA79; AIRM1; CD328; AIRM-1; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1) SIGLEC9 (CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE)

Targeting Strategy

Gene targeting strategy for B-Tg(hSIGLEC7/hSIGLEC9) mice. A BAC containing the entire human SIGLEC7 and SIGLEC9 sequence was randomly inserted into the mouse genome in B-Tg(hSIGLEC7/hSIGLEC9) mice.

Protein Expression Analysis

Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived NK cells were collected from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7-hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.

Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived monocytes were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.

Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived macrophages were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.

Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived granulocytes were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.

Human SIGLEC7 and SIGLEC9 protein expression analysis in B-Tg(hSIGLEC7/hSIGLEC9) mice. Blood-derived T cells were isolated from wild-type C57BL/6 (+/+) and B-Tg(hSIGLEC7/hSIGLEC9) mice, and analyzed by flow cytometry using anti-human SIGLEC7 and anti-human SIGLEC9 antibodies. Human SIGLEC7 and SIGLEC9 protein was exclusively detected in B-Tg(hSIGLEC7/hSIGLEC9) mice compared to wild-type mice.