Basic Information
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Targeting strategy
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Gene targeting strategy for B-Tg(hSIGLEC7/hSIGLEC9) mice. The BAC containing whole human SIGLEC7 and SIGLEC9 genome sequence was inserted into mouse genome randomly in B-Tg(hSIGLEC7/hSIGLEC9) mice.
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Protein expression analysis（F1）
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Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in NK cells.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in monocytes.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in macrophages.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in granulocytes.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in T cells.
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Protein expression analysis（F2）
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Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in NK cells.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in monocytes.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in macrophages.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in granulocytes.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in T cells.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in DC cells.
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Protein expression analysis（F3）
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Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in NK cells.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in monocytes.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in macrophages.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in granulocytes.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were weakly detectable in B-Tg(hSiglec7-hSiglec9) mice in T cells.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were exclusively detectable in B-Tg(hSiglec7/hSiglec9) mice in DC cells.
Strain specific Siglec7 and Siglec9 expression analysis in B-Tg(hSiglec7/hSiglec9) mice by flow cytometry. Blood was collected from wild-type C57BL/6 mice (+/+) and B-Tg(hSiglec7/hSiglec9) mice, and analyzed by flow cytometry with species-specific anti-hSiglec-7 antibody and anti-hSiglec-9 antibody. Human Siglec-7 and Siglec-9 were weakly detectable in B-Tg(hSiglec7-hSiglec9) mice in B cells.
