Basic Information

Common Name
B-hPD-L1 MC38
CD274, PDCD1L1
Catalog Number
Cell Type/Disease
Colon Carcinoma
Mus musculus, C57BL/6


The mouse Pdl1 gene was replaced by human PD-L1 coding sequence in B-hPD-L1 MC38 cells. Human PD-L1 is highly expressed on the surface of B-hPD-L1 MC38 cells.

Gene Targeting Strategy

The exogenous promoter and coding sequence of human PD-L1 was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Pdl1 gene, resulting in a non-functional transcript.

Protein Expression Analysis

PD-L1 expression analysis in humanized B-hPD-L1 MC38 cells. Single cell suspensions from wild-type MC38 and B-hPD-L1 MC38 cultures were stained with species-specific anti-PD-L1 antibodies and analyzed by flow cytometry. Human PD-L1 protein was exclusively detected on the surface of B-hPD-L1 MC38 cells compared to wild-type MC38 cells. The D1 clone of B-hPD-L1 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hPD-L1 MC38 cells. B-hPD-L1 MC38 cells (1×106, 5×105 and 2×105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.


Hou X, Liu S, Zeng Z, Wang Z, Ding J, Chen Y, Gao X, Wang J, Xiao G, Li B, Zhu H, Yang Z. Preclinical imaging evaluation of a bispecific antibody targeting hPD1/CTLA4 using humanized mice. Biomed Pharmacother. 2024 Apr 26;175:116669. PMID: 38677243.

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