B-hCDH17 B16-F10

Basic Information

Description

The mouse Cdh17 gene was replaced by human CDH17 coding sequence in B-hCDH17 B16-F10 cells. Human CDH17 is highly expressed on the surface of B-hCDH17 B16-F10 cells.

Targeting strategy

Gene targeting strategy for B-hCDH17 B16-F10 cells. The exogenous promoter and human CDH17 coding sequence was inserted to replace part of murine exon 3.

Protein expression analysis

CDH17 expression analysis in B-hCDH17 B16-F10 cells by flow cytometry. Single cell suspensions from wild-type B16-F10 and B-hCDH17 B16-F10 cultures were stained with anti-CDH17 antibody (provided by a corporate partner). Human CDH17 was detected on the surface of B-hCDH17 B16-F10 cells but not wild-type B16-F10 cells.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hCDH17 B16-F10 cells. B-hCDH17 B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCDH17 B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies.

Subcutaneous homograft tumor growth of B-hCDH17 B16-F10 cells. B-hCDH17 B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into B-hCDH17 mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCDH17 B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies.

Subcutaneous homograft tumor growth of B-hCDH17 B16-F10 cells. B-hCDH17 B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into C57BL/6 mice or B-hCD3E/h4-1BB mice (female, 7 to 8-week-old, n=8) respectively. Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hCDH17 B16-F10 cells were able to establish tumors in vivo.

CDH17 expression analysis in tumor cells

Tumor cells were harvested at the end point of the experiment and assessed for human CDH17 expression by flow cytometry. As shown in the panel, human CDH17 was expressed on the surface of tumor cells (anti-human CDH17 antibody was provided by a corporate partner).