Basic Information
Description
The mouse Erbb2 gene was replaced by human ERBB2 coding sequence in B-hHER2 EL4 cells. Human HER2 is highly expressed on the surface of B-hHER2 EL4 cells.
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Targeting strategy
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Gene targeting strategy for B-hHER2 EL4 cells. The exogenous promoter and human ERBB2 coding sequence was inserted to replace part of murine exon 2 and all of exons 3~7. The insertion disrupts the endogenous murine Erbb2 gene, resulting in a non-functional transcript.
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Protein Expression Analysis
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HER2 expression analysis in B-hHER2 EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hHER2 EL4 cultures were stained with species-specific anti-HER2 antibody. Human HER2 was detected on the surface of B-hHER2 EL4 cells but not wild-type EL4 cells. The 2-H01 clone of B-hHER2 EL4 cells was used for in vivo experiments.
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Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-hHER2 EL4 cells. B-hHER2 EL4 cells (2×105) and wild-type EL4 cells (2×105) were subcutaneously implanted into C57BL/6 mice (female, 6-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.
Subcutaneous homograft tumor growth of B-hHER2 EL4 cells. B-hHER2 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
