Basic Information

Common Name
B-hTF MC38
Aliases
CD142,F3
Catalog Number
311082
Cell Type/Disease
Colon carcinoma
Species/Strain
Mus musculus, C57BL/6
NCBI Gene ID

Description

The mouse TF gene was replaced by the human TF coding sequence in B-hTF MC38 cells. Human TF is highly expressed on the surface of B-hTF MC38 cells.

Application

B-hTF MC38 cells have the capability to establish tumors in vivo, which can be used for efficacy studies.

Targeting Strategy

The exogenous promoter and human TF coding sequence was inserted to replace part of murine exon 3 and all of exons 4~5. The insertion disrupts the endogenous murine TF gene, resulting in a non-functional transcript.

Protein Expression Analysis

Pre-inoculation

TF expression analysis in B-hTF MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hTF MC38 cultures were stained with species-specific anti-TF antibodies. Human TF was detected on the surface of B-hTF MC38 cells but not wild-type MC38 cells. The 2-D03 clone of B-hTF MC38 cells was used for in vivo experiments.

Post-inoculation

B-hTF MC38 cells were subcutaneously implanted into C57BL/6 mice (n=5). Tumor cells were harvested on day 31 post inoculation and assessed for TF expression by flow cytometry using species-specific antibodies. As shown, human TF was highly expressed on the surface of humanized B-hTF MC38 tumor cells, indicating B-hTF MC38 cells can be used for in vivo efficacy studies of novel TF therapeutics.

Tumor Growth Curve & Body Weight Changes

Tumor Growth Curve & Body Weight Changes in C57BL/6 Mice

Subcutaneous homograft tumor growth of B-hTF MC38 cells. B-hTF MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). (A) Average tumor volume ± SEM and (B) body weight (mean ± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hTF MC38 cells were able to establish tumors in vivo, which can be used for efficacy studies.