Basic Information
B-Tg(hMICA) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Description
The coding sequence of human MICA CDS was randomly inserted into the genome of B-Tg(hMICA) MC38. Human MICA is highly expressed on the surface of B-Tg(hMICA) MC38.
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Protein expression analysis
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Human MICA expression analysis in B-Tg(hMICA) MC38 by flow cytometry. Single cell suspensions from wild-type MC38 and B-Tg(hMICA) MC38 cultures were stained with species-specific anti-MICA antibody. Human MICA was detected on the surface of B-Tg(hMICA) MC38 cells but not wild-type MC38 cells. The 2-G12 clone of B-Tg(hMICA) MC38 cells was used for in vivo experiments. -
Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-Tg(hMICA) MC38 cells. B-Tg(hMICA) MC38 cells (5×105) were subcutaneously implanted into B-hNKG2D mice (male, 7-week-old, n=6). Tumor volume and body weight were measured three times a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(hMICA) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
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Protein expression analysis of tumor cells-shedding MICA(sMICA)
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Shed MICA detection in the cell supernatant and tumor cells. Shed MICA was detected in the cell supernatant derived from B-Tg(hMICA) MC38 cells but not the K562 and MC38 cells(A). Shed MICA was detected in the tumor tissues derived from the 2-G12 clone but not the MC38 cells(B).
