B-NDG hIL15 mice

Basic Information

Strain Name
NOD-Prkdcscid Il2rgtm1Bcgen Il15tm1(IL15)Bcgen/Bcgen
Common Name
B-NDG hIL15 mice
Background
B-NDG
Catalog number
110600
Related Genes
Il15 (interleukin 15)

Description

Il15 (interleukin 15) encodes a a pleiotropic cytokine of the interleukin family of proteins that plays important roles in the innate and adaptive cell homeostasis, as well as peripheral immune function.

It has been reported that IL-15 supports innate lymphoid cell development [1]. Studies using IL-15 transgenic mice[2] and IL-15 knockout mice[3] have show IL-15 to be essential in the development of NK cells, natural killer T (NKT) cells, and memory CD8+ T cells and analyses of engrafted with human HSCs mouse model showed that hIL-15 is required for NK cell development[4].

Biocytogen established a humanized IL15 mice on the background of B-NDG mice (B: Biocytogen; N: NOD background; D: DANPK (Prkdc) null; G: Il2rg knockout.), Is a powerful tool for human immune system reconstituted.

Targeting strategy

 

Details

B-NDG hIL15 Mice are Well Suited for Human Immune System Engraftment

CD34+ constitution standard:hCD45%≥25%

Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. B-NDG hIL15 mice show a significantly higher percentage of human CD45+ cells and of multi-lineage cells,including CD3+ T cells, CD19+ B cells and NK cells. Our results suggest that human B, NK and T cells in reconstituted B-NDG mice were successfully propagated.

Analysis of functions of NK cells in vivo-expanded in B-NDG hIL15 mice after human immune system engraftment.

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hIL15 and B-NDG mice (6 week-old, n=5). All mice were treated with 1.6gry-irradiation. Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. B-NDG hIL15 show a higher percentage of human CD45+  compared with B-NDG. Our results suggest that human leukocytes in reconstituted B-NDG IL15 mice were successfully propagated.

Analysis of functions of NK cells in vivo-expanded in B-NDG hIL15 mice after human immune system engraftment.

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hIL15 and B-NDG mice (6 week-old, n=5). All mice were treated with 1.6gry-irradiation. Representative flow cytometric analysis of PBMCs from mice at 6 week (A) and 8 week (B) after engraftment with human CD34+ cells. B-NDG hIL15 show a higher percentage of human NK cells and the human NK cells express functional proteins.

Analysis of functions of NK cells in vivo-expanded in B-NDG hIL15 mice after human immune system engraftment.

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hIL15 and B-NDG mice (6 week-old, n=5). All mice were treated with 1.6gry-irradiation. Representative flow cytometric analysis of PBMCs from mice at 6 week after engraftment with human CD34+ cells. B-NDG hIL15 show a higher percentage of human NK cells and the human NK cells express functional proteins.

Analysis of functions of NK cells in vivo-expanded in B-NDG hIL15 mice after human immune system engraftment.

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hIL15 and B-NDG mice (6 week-old, n=5). All mice were treated with 1.6gry-irradiation. Representative flow cytometric analysis of PBMCs from mice at 8 week after engraftment with human CD34+ cells. B-NDG hIL15 show a higher percentage of human NK cells and the human NK cells express functional proteins.

 

References

1.Ali, A. K., Nandagopal, N. & Lee, S. H. IL-15-PI3K-AKT-mTOR: A Critical Pathway in the Life Journey of Natural Killer Cells. Frontiers in immunology 6, 355, doi:10.3389/fimmu.2015.00355 (2015).

2.Fehniger, T. A. et al. Fatal leukemia in interleukin 15 transgenic mice follows early expansions in natural killer and memory phenotype CD8+ T cells. The Journal of experimental medicine 193, 219-231, doi:10.1084/jem.193.2.219 (2001).

3.Kennedy, M. K. et al. Reversible defects in natural killer and memory CD8 T cell lineages in interleukin 15-deficient mice. The Journal of experimental medicine 191, 771-780, doi:10.1084/jem.191.5.771 (2000).

4.Matsuda, M. et al. Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice. Life science alliance 2, doi:10.26508/lsa.201800195 (2019).

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