B-NDG hTHPO mice

Basic Information

Strain Name
B-NDG-Thpotm1(THPO)/Bcgen
Common Name
B-NDG hTHPO mice
Background
B-NDG
Catalog number
110590
Related Genes
THPO  (thrombopoietin)

Description

human HSC (hematopoietic stem cells ) engrafted humanized mice are a powerful model allows researchers to examine the human immune system and related primates’ alien virus research and cellular immunotherapy preclinical evaluation. However, maintenance, differentiation, and function of human hematopoietic cells are suboptimal in these hosts. Thrombopoietin (THPO) has been demonstrated as a crucial cytokine supporting maintenance and self-renewal of HSCs.

Biocytogen developed the B-NDG hTHPO mice in which we replaced the gene encoding mouse Thpo by its human CDS region. This mouse combines a B-NDG mouse background and expresses human THPO protein. Homozygous humanization of THPO led to increased levels of human engraftment in the bone marrow of the hosts, and multilineage differentiation of hematopoietic cells was improved, with an increased ratio of myelomonocytic verus lymphoid lineages. Moreover, maintenance of human stem and progenitor cells was improved, as demonstrated by serial transplantation. Therefore, B-NDG hTHPO mice represent a useful model to study human hematopoiesis in vivo.

Details

B-NDG hTHPO mice are well suited for human immune system engraftment

 

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hTHPO and B-NDG mice (female, 6 week-old, n=20). B-NDG mice were treated with 2gry-irradiation, B-NDG hTHPO mice were not irradiated. Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. B-NDG hTHPO mice show a higher percentage of survival rate compared with B-NDG.Non-irradiated B-NDG hTHPO mice showed no difference in the percentage of human CD45+ cells compared to B-NDG mice. The results showed that the human HSC (hematopoietic stem cells) engrafted humanized mice model was successfully constructed.

 

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hTHPO and B-NDG mice (female, 6 week-old, n=20). B-NDG mice were treated with 2gry-irradiation, B-NDG hTHPO mice were not irradiated. Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. Non-irradiated B-NDG hTHPO mice showed no difference in the percentage of human multi-lineage cells compared to B-NDG mice, including T ,B, NK, myeloid cells,monocyte and neutrophils. The results showed that the human HSC (hematopoietic stem cells) engrafted humanized mice model was successfully constructed.

 

 

References

1.Kuter, D. J. The biology of thrombopoietin and thrombopoietin receptor agonists. International journal of hematology 98, 10-23, doi:10.1007/s12185-013-1382-0 (2013).

2.Rongvaux A, et al., Human thrombopoietin knockin mice efficiently support human hematopoiesis in vivo. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2378-83

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