Basic Information
Description
Immunodeficient B-NDG mice were generated by deleting the IL2rg gene from NOD-scid mice. This mouse model has the highest degree of immunodeficiency and is most suitable for engraftment and growth of human tumor cells or tissues.
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Details
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B-NDG hTHPO mice are well suited for human immune system engraftment
Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hTHPO and B-NDG mice (female, 6 week-old, n=20). B-NDG mice were treated with 2gry-irradiation, B-NDG hTHPO mice were not irradiated. Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. B-NDG hTHPO mice show a higher percentage of survival rate compared with B-NDG.Non-irradiated B-NDG hTHPO mice showed no difference in the percentage of human CD45+ cells compared to B-NDG mice. The results showed that the human HSC (hematopoietic stem cells) engrafted humanized mice model was successfully constructed.
Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hTHPO and B-NDG mice (female, 6 week-old, n=20). B-NDG mice were treated with 2gry-irradiation, B-NDG hTHPO mice were not irradiated. Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. Non-irradiated B-NDG hTHPO mice showed no difference in the percentage of human multi-lineage cells compared to B-NDG mice, including T ,B, NK, myeloid cells，monocyte and neutrophils. The results showed that the human HSC (hematopoietic stem cells) engrafted humanized mice model was successfully constructed.
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References
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1.Kuter, D. J. The biology of thrombopoietin and thrombopoietin receptor agonists. International journal of hematology 98, 10-23, doi:10.1007/s12185-013-1382-0 (2013).
2.Rongvaux A, et al., Human thrombopoietin knockin mice efficiently support human hematopoiesis in vivo. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2378-83
