Basic Information

Strain Name
NOD.CB17-PrkdcscidIl2rgtm1Thpotm1(THPO)/Bcgen
Common Name
B-NDG hTHPO mice
Background
B-NDG
Catalog number
110590
Related Genes
THPO  (thrombopoietin)
NCBI Gene ID

Description

Immunodeficient B-NDG mice were generated by deleting the IL2rg gene from NOD-scid mice. This mouse model has the highest degree of immunodeficiency and is most suitable for engraftment and growth of human tumor cells or tissues.

Details

B-NDG hTHPO mice are well suited for human immune system engraftment

 

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hTHPO and B-NDG mice (female, 6 week-old, n=20). B-NDG mice were treated with 2gry-irradiation, B-NDG hTHPO mice were not irradiated. Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. B-NDG hTHPO mice show a higher percentage of survival rate compared with B-NDG.Non-irradiated B-NDG hTHPO mice showed no difference in the percentage of human CD45+ cells compared to B-NDG mice. The results showed that the human HSC (hematopoietic stem cells) engrafted humanized mice model was successfully constructed.

 

Human CD34+ cells(0.15M) were intravenous implanted into homozygote B-NDG hTHPO and B-NDG mice (female, 6 week-old, n=20). B-NDG mice were treated with 2gry-irradiation, B-NDG hTHPO mice were not irradiated. Representative flow cytometric analysis of PBMCs from mice after engraftment with human CD34+ cells. Non-irradiated B-NDG hTHPO mice showed no difference in the percentage of human multi-lineage cells compared to B-NDG mice, including T ,B, NK, myeloid cells,monocyte and neutrophils. The results showed that the human HSC (hematopoietic stem cells) engrafted humanized mice model was successfully constructed.

 

 

References

1.Kuter, D. J. The biology of thrombopoietin and thrombopoietin receptor agonists. International journal of hematology 98, 10-23, doi:10.1007/s12185-013-1382-0 (2013).

2.Rongvaux A, et al., Human thrombopoietin knockin mice efficiently support human hematopoiesis in vivo. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2378-83