Basic Information

Strain Name
NOD.CB17-PrkdcscidIL2rgtm1Il3tm1(IL3)Csf2tm1(CSF2)Csf1tm1(CSF1)Thpotm1(THPO)/Bcgen
Common Name
B-NDG MGMT3 mice
Background
B-NDG
Catalog number
111882
Aliases
IL3: IL-3, MCGF, MULTI-CSF Csf2: CSF, GMCSF Csf1: CSF-1, MCSF THPO: MGDF, MKCSF, ML, MPLLG, THCYT1, TPO
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-NDG MGMT3 mice. The full sequences of mouse Il3, Csf2, Csf1, Thpo genes except the UTRs were respectively replaced with the coding sequences (CDS) of human IL3, CSF2, CSF1, THPO genes.

Protein expression analysis

Species-specific GM-CSF, CSF1 and THPO protein expression analysis in wild-type B-NDG and B-NDG MGMT3 mice. Following LPS stimulation in vivo, serum was collected from wild-type B-NDG (+/+) and homozygous B-NDG MGMT3 (H/H) mice and analyzed by ELISA (n=3) using species-specific kits. Murine GM-CSF, CSF1 and THPO proteins were detected in B-NDG mice, while human GM-CSF, CSF1 and THPO proteins were detected in B-NDG MGMT3 mice. Mouse and/or human IL3 protein expression was not detected in immunodeficient B-NDG and/or B-NDG MGMT3 mice due to the lack of mature (activated) T cells.

Human CD34+ HSC engraftment for human immune system reconstitution

Human CD34+ HSC engraftment for human immune system reconstitution. Wild-type B-NDG and B-NDG MGMT3 (both sex, 24-72 hr after birth, n=15) mice were engrafted with human CD34+ HSCs (3E4) by intravenous (temporal vein) injection. B-NDG mice were treated with 1.0 Gy-irradiation, while B-NDG MGMT3 mice were not irradiated. (A) Survival rates were analyzed by Kaplan Meier survival curves. (B) Body weight measurements. Results showed that the survival rate of B-NDG MGMT3 mice were similar to that of B-NDG mice until 16 weeks post human CD34+ HSC engraftment, while body weight of B-NDG MGMT3 mice was significantly higher than that of B-NDG mice. Values are expressed as mean ± SEM. HSCs: hematopoietic stem cells.

Human CD34+ HSC engraftment for human immune system reconstitution. Wild-type B-NDG and B-NDG MGMT3 (both sex, 24-72 hr after birth, n=15) mice were engrafted with human CD34+ HSCs (3E4) by intravenous (temporal vein) injection. B-NDG mice were treated with 1.0 Gy-irradiation, while B-NDG MGMT3 mice were not irradiated. (A, B) Flow cytometry analysis of peripheral blood lymphocytes. Results showed that the proportion of CD45+ cells in B-NDG MGMT3 mice reached 25% starting from 12 weeks after engraftment and continued to rise, significantly higher than that in B-NDG mice. The proportions of monocytes, MDSCs, DCs and Tregs in B-NDG MGMT3 mice were higher than that in B-NDG mice. Values are expressed as mean ± SEM.

Note: Data from week 18 in Panel A was not meaningful due to flow cytometry problems.