Basic Information

Strain Name
NOD.CB17-Prkdcscid Il2rgtm1Bcgen Kittm1(V831M)Bcgen/Bcgen
Common Name
B-NDG Kit W41 mice
Background
B-NDG
Catalog number
112914
Aliases 
PBT, SCFR, C-Kit, CD117, MASTC
NCBI Gene ID
Application Fields

This product can accept human hematopoietic stem cell transplantation without irradiation, making it suitable for research in the fields of blood and immunity.

mRNA expression analysis

Strain specific analysis of Kit mRNA expression in B-NDG mice and B-NDG Kit W41 mice by RT-PCR. Spleen and lung RNA was isolated from B-NDG mice (+/+) and heterozygous B-NDG Kit W41 mice (Mut/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Kit primers. Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.

Protein expression analysis-Neutrophils

Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in neutrophils of B-NDG mice and homozygous B-NDG Kit W41 mice.

Protein expression analysis-DCs

Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in DCs of B-NDG mice and homozygous B-NDG Kit W41 mice.

Protein expression analysis-Macrophages&Monocytes

Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in CD11b+ cells of B-NDG mice and homozygous B-NDG Kit W41 mice.

Frequency of leukocyte subpopulations

Frequency of leukocyte subpopulations by flow cytometry. Splenocytes, blood cells and bone marrow cells were isolated from B-NDG mice and homozygous B-NDG Kit W41 mice (n=3, 8-week-old). Flow cytometry analysis of the Splenocytes, blood cells and Bone marrow cells were performed to assess the frequency of leukocyte subpopulations. Percentages of T cells, B cells, NK cells, DCs, granulocytes, monocytes, macrophages in B-NDG Kit W41 mice were similar to those in B-NDG mice, demonstrating that mutation of Kit does not change the frequency or distribution of these cell types in spleen(A), blood(B) and bone marrow(C). Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to top