Basic Information
This product can accept human hematopoietic stem cell transplantation without irradiation, making it suitable for research in the fields of blood and immunity.
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mRNA expression analysis
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Strain specific analysis of Kit mRNA expression in B-NDG mice and B-NDG Kit W41 mice by RT-PCR. Spleen and lung RNA was isolated from B-NDG mice (+/+) and heterozygous B-NDG Kit W41 mice (Mut/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Kit primers. Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.
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Protein expression analysis-Neutrophils
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Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in neutrophils of B-NDG mice and homozygous B-NDG Kit W41 mice.
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Protein expression analysis-DCs
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Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in DCs of B-NDG mice and homozygous B-NDG Kit W41 mice.
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Protein expression analysis-Macrophages&Monocytes
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Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in CD11b+ cells of B-NDG mice and homozygous B-NDG Kit W41 mice.
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Frequency of leukocyte subpopulations
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Frequency of leukocyte subpopulations by flow cytometry. Splenocytes, blood cells and bone marrow cells were isolated from B-NDG mice and homozygous B-NDG Kit W41 mice (n=3, 8-week-old). Flow cytometry analysis of the Splenocytes, blood cells and Bone marrow cells were performed to assess the frequency of leukocyte subpopulations. Percentages of T cells, B cells, NK cells, DCs, granulocytes, monocytes, macrophages in B-NDG Kit W41 mice were similar to those in B-NDG mice, demonstrating that mutation of Kit does not change the frequency or distribution of these cell types in spleen(A), blood(B) and bone marrow(C). Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.
