Basic Information
Description
B-ob/ob mice are a powerful model for studying hyperglycemia and obesity.
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Targeting Strategy
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Model validation and analysis
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The results showed that the body weight of homozygous B-ob/ob mice (-/-) was continuously higher than that of the control group after 4 weeks of age.
The results showed that the blood glucose of homozygous B-ob/ob mice (-/-) was continuously higher than that of the control group after 4 weeks of age.
Model validation
Efficacy of the anti-human GCGR antibody drug crotedumab in B-ob/ob mice. ( A) Body weight changes in B-ob/ob mice. (B-C) Non-fasting blood glucose and fasting blood glucose measurement. Male B-ob/ob mice, 8-10 weeks old, were randomly assigned to 2 groups of 6-7 animals each. Dosing occurred on Day 0. Non-fasting blood glucose was measured on days 0, 3, and 7, and fasting blood glucose was measured after 6 hours of fasting. ( D) Crotedumab improves glucose tolerance. E) Area under the curve of blood glucose content. Mice were fasted for 6 h under free access to water, fasting blood glucose was measured at the tail tip (0 min), glucose was injected intraperitoneally at 2 g/kg, and blood glucose was measured at the indicated times. (Fig. F-G) glucagon and insulin measurements. The results showed that crotedumab had hypoglycemic effects in both fasted and non-fasted states, while being able to improve glucose tolerance in B-ob/ob mice. (Fig. Data are mean ± SEM; P ≤ 0.05, P ≤ 0.01, P ≤ 0.001)
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References
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1. Leptin receptor gene polymorphisms and sex modify the association between
acetaminophen use and asthma among young adults: results from two observational studies.Ziyab AH, et al. Respir Res, 2018 Sep 19. PMID 30231898.
2. Food Consumption as a Modifier of the Association between LEPR Gene Variants and Excess Body Weight in Children and Adolescents: A Study of the SCAALA Cohort.Dos Santos Rocha A, et al. Nutrients, 2018 Aug 18. PMID 30126176.
