B-Luc ID8

Basic Information

Description

This B-Luc ID8 cell line expresses firefly luciferase as a marker of ID8 cells. Fluorescence could be detected in B-Luc ID8 cells.

In vitro luciferase activity of B-Luc ID8 cells

Luminescence signal intensity of B-Luc ID8 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc ID8 cells have a strong luminescence signal. The mix clone and 2-H02 of B-Luc ID8 cells can be used in vivo experiments.

Quantitative imaging data & body weight changes

B-Luc ID8 cells (5×10⁵, 1×10⁶) were injected into the ovary of wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc ID8 cells can be used for in vivo efficacy evaluation. Note: The fluorescence signal on the 14th day decreased because the mouse hair was not removed completely.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Luc ID8 cells. B-Luc ID8 cells (1×10⁶) and wild-type ID8 cells (1×10⁶) were subcutaneously implanted into C57BL/6 mice (female, 7-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Luc ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

Subcutaneous homograft tumor growth of B-Luc ID8 cells. B-Luc ID8 cells (1×10⁶) and wild-type ID8 cells (1×10⁶) were subcutaneously implanted into C57BL/6 mice (female, 5-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Luc ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve of individual mice

B-Luc ID8 tumor growth of individual mice. B-Luc ID8 cells (1×10⁶) and wild-type ID8 cells (1×10⁶) were subcutaneously implanted into C57BL/6 mice (female, 7-8-week-old, n=5). As shown in panel, B-Luc ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

B-Luc ID8 tumor growth of individual mice. B-Luc ID8 cells (1×10⁶) and wild-type ID8 cells (1×10⁶) were subcutaneously implanted into C57BL/6 mice (female, 5-week-old, n=6). As shown in panel, B-Luc ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

Quantitative imaging data & body weight changes

Tumor growth and in vivo imaging of B-Luc ID8 cells. B-Luc ID8 cells (1×10⁶) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc ID8 cells can be used for in vivo efficacy evaluation.