Basic Information

Common Name
B-Tg(Luc) HCT 116
Catalog Number
Colorectal carcinoma
Tissue types
B-Tg(Luc) HCT 116 cell line can be used to evaluate anti-cancer drugs in colon cancer in vivo. It can be applied subcutaneously and orthotopically to colons in mice.


This B-Tg(Luc) HCT 116 cell line expresses firefly luciferase as a marker of HCT 116 cells. Fluorescence could be detected in B-Tg(Luc) HCT 116 cells.

Targeting strategy

Gene targeting strategy for B-Tg(Luc) HCT 116 cells. The exogenous promoter and luciferase coding sequence were inserted into the mouse genome randomly.

Quantitative imaging data & body weight changes

B-Tg(Luc) HCT 116 cells (1×105, 5×105 ,1×106) were injected into the colon of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Tg(Luc) HCT 116 cells can be used for in vivo efficacy evaluation.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(Luc) HCT 116 cells. B-Tg(Luc) HCT 116 cells (5×106) were subcutaneously implanted into B-NDG mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(Luc) HCT 116 cells were able to establish tumors in vivo and can be used for efficacy studies.

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