Basic Information
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Description
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This B-Tg(Luc) HCT 116 cell line expresses firefly luciferase as a marker of HCT 116 cells. Fluorescence could be detected in B-Tg(Luc) HCT 116 cells.
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Targeting strategy
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Gene targeting strategy for B-Tg(Luc) HCT 116 cells. The exogenous promoter and luciferase coding sequence were inserted into the mouse genome randomly.
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Quantitative imaging data & body weight changes
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B-Tg(Luc) HCT 116 cells (1×105, 5×105 ,1×106) were injected into the colon of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Tg(Luc) HCT 116 cells can be used for in vivo efficacy evaluation.
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Imaging analysis in vivo
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HE staining
Histopathological HE staining was performed in two suspected tumor metastasis tissue samples. The test results showed that tumor metastasis occurred in the pancreas of mice.
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Tumor growth curve & Body weight changes
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Subcutaneous homograft tumor growth of B-Tg(Luc) HCT 116 cells. B-Tg(Luc) HCT 116 cells (5×106) were subcutaneously implanted into B-NDG mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(Luc) HCT 116 cells were able to establish tumors in vivo and can be used for efficacy studies.
