Basic Information
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In vitro luciferase activity of B-Tg(Luc) U-87 MG cells
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Luminescence signal intensity of B-Tg(Luc) U-87 MG cells. Single cell suspensions from wild-type U-87 MG and B-Tg(Luc) U-87 MG #1-D02 cultures were measured using the Bright-GloTM luciferase Assay (Promega, Catalog No. E4030). B-Tg(Luc) U-87 MG cells have a strong luminescence signal that is not present in wild-type U-87 MG cells.
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Tumor growth curve & body weight changes
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Subcutaneous tumor growth of B-Tg(Luc) U-87 MG cells. B-Tg(Luc) U-87 MG cells (5×106) and wild-type U-87 MG cells (5×106) were subcutaneously implanted into B-NDG mice (female, 7-9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. Results indicate that B-Tg(Luc) U-87 MG cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
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Quantitative imaging data
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Growth kinetics of B-Tg(Luc) U-87 MG tumors determined by bioluminescence imaging (BLI) . B-Tg(Luc) U-87 MG cells (5×106) were subcutaneously implanted into B-NDG mice (female, 7-9-week-old, n=6). Signal intensity and body weight were measured twice a week. (A) Signal intensity. (B) Raw bioluminescence images. These results indicate that B-Tg(Luc) U-87 MG cells can be used for in vivo efficacy evaluation. Values are expressed as mean ± SEM.
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Quantitative imaging data & body weight changes
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Growth kinetics of B-Tg(Luc) U-87 MG tumors determined by bioluminescence imaging (BLI) . B-Tg(Luc) U-87 MG cells (1×105, 2×105) were injected into the brain of wild-type B-NDG mice (female, 8-week-old, G1 n=5, G2 n=6). Signal intensity and body weight were measured twice a week. (A) Signal intensity. (B) Body weight. (C) Raw bioluminescence images. These results indicate that B-Tg(Luc) U-87 MG cells can be used for in vivo efficacy evaluation in brain orthotopic tumor models. Values are expressed as mean ± SEM.
