Basic Information

Common name
B-Tg(mCcl2) MC38
Catalog number
322119
Aliases
JE, HC11, MCAF, MCP1, MCP-1, Scya2, Sigje, SMC-CF
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue types
Colon
Tissue
Colon
Application
B-Tg(mCcl2) MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Description

The exogenous promoter and mouse Ccl2 coding sequence were inserted into the mouse genome randomly. Mouse Ccl2 can be secreted by B-Tg(mCcl2) MC38 cells.

Targeting strategy

Gene targeting strategy for B-Tg(mCcl2) MC38 cells. The exogenous promoter and mouse Ccl2 coding sequence were inserted into the mouse genome randomly.

Protein Expression Analysis

Ccl2 expression analysis in B-Tg(mCcl2) MC38 cells by ELISA. Mouse Ccl2 was detected in the supernatant of B-Tg(mCcl2) MC38 cells. The 1-C06 clone of B-Tg(mCcl2) MC38 cells was used for in vivo experiments

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Tg(mCcl2) MC38 cells. B-Tg(mCcl2) MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mCcl2) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Mccl2 expression analysis in tumor

Tumor cells were harvested at the end of experiment and assessed for mouse Ccl2 expression by ELISA. As shown, mouse Ccl2 was highly expressed in the tumor homogenate. Data was shown as Mean±SEM, and analyzed using T tests compared with G1. (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001)

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