Basic Information
ADAMTS5: ADAM-TS 11, ADAM-TS 5, ADAM-TS5, ADAMTS-11, ADAMTS-5, ADAMTS11, ADMP-2
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mRNA expression analysis
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Strain specific analysis of ADAMTS5 mRNA expression in wild-type C57BL/6 mice and B-hADAMTS5 mice by RT-PCR. Testis RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADAMTS5 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADAMTS5 primers. Mouse Adamts5 mRNA was detectable only in wild-type C57BL/6 mice. Human ADAMTS5 mRNA was detectable only in homozygous B-hADAMTS5 mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of ADAMTS5 protein expression in homozygous B-hADAMTS5 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADAMTS5 mice (H/H) , and then analyzed by western blot with anti-ADAMTS5 antibody. 40 μg total proteins were loaded for western analysis. The antibody cross recognizes human and mouse ADAMTS5. ADAMTS5 was detected in kidney, testis, lung, spleen, and brain.
