Basic Information
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Targeting strategy
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Gene targeting strategy for B-hAMIGO2 mice.
The exon 2 of mouse Amigo2 gene that encode the extracellular domain were replaced by human AMIGO2 exon 3 in B-hAMIGO2 mice. -
Protein expression analysis
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Western blot analysis of AMIGO2 protein expression in homozygous B-hAMIGO2 mice. Various tissue lysates were collected from C57BL/6 wildtype (+/+) mice and homozygous B-hAMIGO2 (H/H) mice, and then analyzed by western blot with anti-AMIGO2 antibody. 40 μg total proteins were loaded for western blotting analysis. AMIGO2 was detected in lung, kidney, stomach and spleen.
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mRNA expression analysis
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Strain specific analysis of AMIGO2 mRNA expression in wild-type C57BL/6 mice and B-hAMIGO2 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hAMIGO2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human AMIGO2 primers. Mouse Amigo2 mRNA was detectable only in wild-type C57BL/6 mice. Human AMIGO2 mRNA was detectable only in homozygous B-hAMIGO2 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Amigo2 mRNA was detectable in lung of wild-type C57BL/6 mice. Human AMIGO2 mRNA was detectable in B-hAMIGO2 mice.Protein expression analysis:
AMIGO2 was detectable in wild-type C57BL/6 mice and homozygous B-hAMIGO2 mice.
