Basic Information
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Protein expression analysis
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Western blot analysis of APLP2 protein expression in homozygous B-hAPLP2 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAPLP2 mice (H/H), and then analyzed by western blot with anti-APLP2 antibody. 40 μg total proteins were loaded for western blotting analysis. hAPLP2 was detected in heart, liver, spleen, lung, kidney and small intestine from homozygous mice.
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mRNA expression analysis
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Strain specific analysis of APLP2 mRNA expression in wild-type C57BL/6 mice and B-hAPLP2 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hAPLP2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APLP2 primers. Mouse Aplp2 mRNA were detectable only in wild-type C57BL/6 mice. Human APLP2 mRNA was detectable only in homozygous B-hAPLP2 mice but not in wild-type mice.
