Basic Information

Strain Name
C57BL/6N-Apoc3tm1(APOC3)/Bcgen
Common Name
B-hAPOC3 mice
Background
C57BL/6N
Catalog Number
112411
NCBI Gene ID
Related Genes
APOC3: Apo-C3, ApoC-3, APOCIII

Targeting strategy

Gene targeting strategy for B-hAPOC3 mice.
The exons 2~4 of mouse Apoc3 gene that encodes the full-length protein was replaced by human APOC3 exons 2~4 in B-hAPOC3 mice.

Protein expression analysis

Strain specific APOC3 expression analysis in homozygous B-hAPOC3 mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hAPOC3 mice (H/H) and analyzed by ELISA with species-specific APOC3 ELISA kit. Human APOC3 was exclusively detectable in homozygous B-hAPOC3 mice but not in wild-type mice.

mRNA expression analysis

Strain specific analysis of APOC3 mRNA expression in wild-type C57BL/6 mice and B-hAPOC3 mice by RT-PCR. Liver RNA were isolated from wild type C57BL/6 mice (+/+) and homozygous B-hAPOC3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOC3 primers. Mouse Apoc3 mRNA was detectable only in wild-type C57BL/6 mice. Human APOC3 mRNA was detectable only in homozygous B-hAPOC3 mice but not in wild-type mice.

Summary

mRNA expression analysis:
Mouse Apoc3 mRNA was detectable in liver of wild-type C57BL/6 mice. Human APOC3 mRNA was detectable in B-hAPOC3 mice.

Protein expression analysis:
Human APOC3 was exclusively detectable in homozygous B-hAPOC3 mice but not in wild-type mice.

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