Basic Information

Strain name
C57BL/6JNifdc-Apoetm1(APOE*3)Bcgen/Bcgen
Common name
B-hAPOE3 mice
Background
C57BL/6JNifdc
Catalog number
112561
Aliases
APOE, AD2, APO-E, LDLCQ5, LPG
NCBI gene ID
Application
This product is used as the metabolic disease models associated with APOE3 or as a control for pharmacodynamics and safety evaluation of Alzheimer’s disease drugs in B-hAPOE4 mice.

Targeting strategy

Gene targeting strategy for B-hAPOE3 mice.

The exons 2~4 of mouse Apoe gene that encode the full-length protein were replaced by human APOE3 exons 2~4 in B-hAPOE3 mice.

mRNA expression analysis

Strain specific analysis of APOE mRNA expression in wild-type C57BL/6J mice and B-hAPOE3 mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6J mice (+/+) and homozygous B-hAPOE3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOE primers. Mouse Apoe mRNA was detectable only in wild-type C57BL/6 mice. Human APOE mRNA was detectable only in homozygous B-hAPOE3 mice but not in wild-type mice.

Protein expression analysis in serum

Strain specific APOE expression analysis in homozygous B-hAPOE3 mice by ELISA. Serum collected from wild-type C57BL/6J mice (+/+) and homozygous B-hAPOE3 mice (H/H) were analyzed by ELISA with species-specific APOE ELISA kit. Mouse APOE was detectable in wild-type mice. Human APOE was exclusively detectable in homozygous B-hAPOE3 mice but not in wild-type mice.

Protein expression analysis in brain

Strain specific APOE expression analysis in homozygous B-hAPOE3 mice by ELISA. Whole brain lysate collected from wild-type C57BL/6J mice (+/+) and homozygous B-hAPOE3 mice (H/H). APOE was measured in the brain homogenates using species-specific APOE ELISA kit and then normalized to total protein levels. Mouse APOE was detectable in wild-type mice. Human APOE was exclusively detectable in homozygous B-hAPOE3 mice but not in wild-type mice.

Back to top
WordPress Double Opt-in by Forge12