Basic Information
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Protein expression analysis
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Western blot analysis of CD151 protein expression in homozygous B-hCD151 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD151 mice (H/H), and then analyzed by western blot with species-specific anti-CD151 antibody. 30 μg total protein was loaded for western blotting analysis. hCD151 was exclusively detected in colon, kidney, lung and spleen in homozygous B-hCD151 mice.
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mRNA expression analysis
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Strain specific analysis of CD151 mRNA expression in wild-type C57BL/6 mice and B-hCD151 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCD151 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CD151 primers. Mouse Cd151 mRNA was detectable only in wild-type C57BL/6 mice. Human CD151 mRNA was detectable only in homozygous B-hCD151 mice.
