Basic Information

Strain Name
C57BL/6-Cd1dtm1(CD1D)/Bcgen
Common Name
B-hCD1D mice
Background
C57BL/6N
Catalog number
111558
Aliases
R3G1, CD1A, R3
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD1D mice. Exon 1~6 of mouse Cd1d gene that encodes the full-length protein was replaced by human CD1D exon 2~7 in B-hCD1D mice.

mRNA expression analysis

Species-specific CD1D gene expression analysis in wild-type and humanized B-hCD1D mice by RT-PCR. Murine Cd1d mRNA was detected in splenocytes isolated from wild-type C57BL/6 (+/+) mice, while human CD1D mRNA was detected in homozygous B-hCD1D (H/H) mice.

Protein expression analysis

Species-specific CD1D protein expression analysis in wild-type and humanized B-hCD1D mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD1D (H/H) mice and analyzed by flow cytometry using species-specific anti-CD1D antibodies. Murine CD1D protein was detected in wild-type mice, while human CD1D protein was detected in B-hCD1D mice.

Back to top