Basic Information
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mRNA expression analysis
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Strain specific analysis of CD300A gene expression in wild type C57BL/6 mice and homozygous B-hCD300A mice by RT-PCR. Splenocytes were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H). Mouse CD300a mRNA was detectable only in wild type C57BL/6 mice. Human CD300A mRNA was detectable only in homozygous B-hCD300A mice, but not in wild type C57BL/6 mice.
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Protein expression analysis
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Strain specific CD300A expression analysis in homozygous B-hCD300A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H), and analyzed by flow cytometry with anti-hCD300A antibody. Human CD300A was detectable in B cells, NK cells and DCs in homozygous B-hCD300A mice (H/H).
Strain specific CD300A expression analysis in homozygous B-hCD300A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD300A mice (H/H), and analyzed by flow cytometry with anti-hCD300A antibody. Human CD300A was detectable in B cells, NK cells, DCs, neutrophils, monocytes and macrophages in homozygous B-hCD300A mice (H/H).
