Basic Information

Strain Name
C57BL/6-Cd63tm1(CD63)/Bcgen
Common Name
B-hCD63 mice
Catalog number
111103
Background
C57BL/6
Aliases
CD63(CD63 molecule), LAMP-3, ME491, MLA1, OMA81H, TSPAN30
NCBI Gene ID

Basic information

Signaling Pathways Altered During the Metastatic Progression of Melanoma

•Timp1 interacts with beta-1 integrin and CD63 along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway independently of Akt phosphorylation.

•In the melanocyte lineage melan-a, there is no interaction among CD63, β1-integrin and TIMP1. In the pre-malignant 4C cell line, TIMP1 associates with CD63, but not with β1-integrins, and CD63 interacts with β1-integrins. In 4C11- and 4C11+ melanoma cell lines, the formation of CD63/β1-integrin/TIMP1 complex occurs, which could be related to a more efficient activation of cell survival signals.

Targeting strategy

Gene targeting strategy for B-hCD63 mice.
The exons 2-8 of mouse Cd63 gene that encode the full-length protein were replaced by human CD63 exons 2-8 in B-hCD63 mice.

Details

mRNA expression analysis

Strain specific analysis of CD63 gene expression in wild type (WT) and B-hCD63 mice by RT-PCR.
Mouse Cd63 mRNA was detectable only in kidney of wild type (+/+) mice. Human CD63 mRNA was detectable only in H/H but not in +/+ mice.

Protein expression analysis

Strain specific CD63 expression analysis in homozygous B-hCD63 mice by flow cytometry.
Macrophages were collected from bone marrow of WT and homozygous B-hCD63 mice, and analyzed by flow cytometry with species-specific CD63 antibody. Mouse CD63 was detectable in WT mice. Human CD63 was exclusively detectable in homozygous B-hCD63 mice but not WT mice.

Strain specific CD63 expression analysis in homozygous B-hCD63 mice by flow cytometry.
Monocytes were collected from bone marrow of WT and homozygous B-hCD63 mice, and analyzed by flow cytometry with species-specific CD63 antibody. Mouse CD63 was detectable in WT mice. Human CD63 was exclusively detectable in homozygous B-hCD63 mice but not WT mice.