Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCD63 mice. The exons 2-8 of mouse Cd63 gene that encode the full-length protein were replaced by human CD63 exons 2-8 in B-hCD63 mice.
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mRNA expression analysis
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Species-specific CD63 gene expression analysis in wild-type and humanized B-hCD63 mice by RT-PCR. Murine Cd63 mRNA was detected in kidney tissue isolated from wild-type (+/+) mice, while human CD63 mRNA was detected in B-hCD63 mice.
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Protein expression analysis
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Species-specific CD63 protein expression analysis in wild-type and humanized B-hCD63 mice. Bone marrow-derived macrophages were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD63 (H/H) mice, and analyzed by flow cytometry using species-specific anti-CD63 antibodies. Murine CD63 protein was detected in wild-type mice, while human CD63 protein was exclusively detected in B-hCD63 mice.
Species-specific CD63 protein expression analysis in wild-type and humanized B-hCD63 mice. Bone marrow-derived monocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD63 (H/H) mice, and analyzed by flow cytometry using species-specific anti-CD63 antibodies. Murine CD63 protein was detected in wild-type mice, while human CD63 protein was exclusively detected in B-hCD63 mice.
