Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD8 mice.
The exons 1~3 and partial exon 4 of mouse Cd8a gene that encode the extracellular domain were replaced by human CD8A exons 1~3 and partial exon 4 in B-hCD8 mice. The exons 1~3 and partial exon 4 of mouse Cd8b1 gene that encode the extracellular domain were replaced by human CD8B exons 1~3 and partial exon 4 in B-hCD8 mice.
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Protein expression analysis in blood cells
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Strain specific CD8 expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD8 mice by flow cytometry. Blood were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD8 mice (H/H), respectively, and analyzed by flow cytometry with species-specific anti-hCD8a, anti-hCD8b, anti-mCD8a antibodies. hCD8a and hCD8b were exclusively detectable in homozygous B-hCD8 mice(A). mCD8a was not detectable in homozygous B-hCD8 mice(B).
