Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCLDN1 mice.
The exons 1~4 of mouse Cldn1 gene were replaced by human CLDN1 exons 1~4 in B-hCLDN1 mice.
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Protein expression analysis
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Strain specific CLDN1 expression analysis in homozygous B-hCLDN1 mice by flow cytometry. Liver were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCLDN1 mice (H/H) and analyzed by flow cytometry with anti-hCLDN1 antibody A and B. hCLDN1 was detectable in homozygous B-hCLDN1 mice but not in wild-type C57BL/6 mice.
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mRNA expression analysis
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Strain specific analysis of CLDN1 mRNA expression in wild-type C57BL/6 mice and B-hCLDN1 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCLDN1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CLDN1 primers. Mouse Cldn1 mRNA was detectable only in wild-type C57BL/6 mice. Human CLDN1 mRNA was detectable only in homozygous B-hCLDN1 mice but not in wild-type mice.
