Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCLDN2 mice.
The exon 2 of mouse Cldn2 gene that encodes the full-length protein was replaced by human CLDN2 exon 2 in B-hCLDN2 mice. -
Protein expression analysis
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Strain specific CLDN2 expression analysis in homozygous B-hCLDN2 mice by western blot. Kidney, liver, lung and duodenum were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCLDN2 mice (H/H), and analyzed by western blot with anti-CLDN2 antibody. m/hCLDN2 was detectable in kidney of wild-type C57BL/6 mice and homozygous B-hCLDN2 mice as the antibody is cross-recognize both human and mouse CLDN2.
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mRNA expression analysis
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Strain specific analysis of CLDN2 mRNA expression in wild-type C57BL/6 mice and B-hCLDN2 mice by RT-PCR. Kidney RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hCLDN2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CLDN2 primers. Mouse Cldn2 mRNA was detectable only in wild-type C57BL/6 mice. Human CLDN2 mRNA was detectable only in homozygous B-hCLDN2 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Cldn2 mRNA was detectable in kidney of wild-type C57BL/6 mice. Human CLDN2 mRNA was detectable in B-hCLDN2 mice.Protein expression analysis:
CLDN2 protein was detected in kidney of wild-type C57BL/6 mice and homozygous B-hCLDN2 mice, as the antibody is cross-recognize both human and mouse CLDN2.
