Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCLEC1A mice.
The exons 3~6 of mouse Clec1a gene that encodes the extracellular domain was replaced by human CLEC1A exons 3~6 in B-hCLEC1A mice.
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Protein expression analysis
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Western blot analysis of CLEC1A protein expression in homozygous B-hCLEC1A mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCLEC1A mice (H/H), and then analyzed by western blot with anti-CLEC1A antibody. 40 μg total proteins were loaded for western blotting analysis. hCLEC1A was detected in heart, lung, kidney, small intestine, thymus and lymph node (red arrow).
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mRNA expression analysis
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Strain specific analysis of CLEC1A mRNA expression in wild-type C57BL/6 mice and B-hCLEC1A mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCLEC1A mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CLEC1A primers. Mouse Clec1a mRNA were detectable only in wild-type C57BL/6 mice. Human CLEC1A mRNA was detectable only in homozygous B-hCLEC1A mice but not in wild-type mice.
