Basic Information

Strain Name
C57BL/6-Cspg5tm2(CSPG5)Bcgen/Bcgen
Common Name
B-hCSPG5 mice
Background
C57BL/6
Catalog Number
112235
Aliases
CSPG5: NGC
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCSPG5 mice. The exons 1~3 of mouse Cspg5 gene that encode the extracellular domain were replaced by human CSPG5 exons 1~3 in B-hCSPG5 mice.

Protein expression analysis

Western blot analysis of CSPG5 protein expression in homozygous B-hCSPG5 mice. Cortex lysates were collected from C57BL/6 wild-type (+/+) mice and homozygous B-hCSPG5 mice (H/H), and then analyzed by western blot with species-specific anti-CSPG5 antibody. 40 μg total proteins were loaded for western blotting analysis. CSPG5 protein was detected in the cortex of wild-type C57BL/6 mice and homozygous B-hCSPG5 mice, as the antibody is cross-recognize both human and mouse CSPG5. It is impossible to conclude from the data that the observed three bands are CSPG5 three isoforms.

mRNA expression analysis

Strain specific analysis of CSPG5 mRNA expression in wild-type C57BL/6 mice and B-hCSPG5 mice by RT-PCR. Cortex RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCSPG5 mice(H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CSPG5 primers. Mouse Cspg5 mRNA was detectable only in wild-type C57BL/6 mice. Human CSPG5 mRNA was detectable only in homozygous B-hCSPG5 mice but not in wild-type mice.

Summary

mRNA expression analysis:

Mouse Cspg5 mRNA was detectable in cortex of wild-type C57BL/6 mice. Human CSPG5 mRNA was detectable in B-hCSPG5 mice.

Protein expression analysis:

CSPG5 protein was detected in the cortex of wild-type C57BL/6 mice and homozygous B-hCSPG5 mice, as the antibody is cross-recognize both human and mouse CSPG5.

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