Basic Information
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Protein expression analysis
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Western blot analysis of DPP3 protein expression in homozygous B-hDPP3 mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hDPP3 mice (H/H), and then analyzed by western blot with species-specific anti-DPP3 antibody. 40 μg total proteins were loaded for western blotting analysis. hDPP3 was detected in liver,kidney,heart,lung,spleen and thymus from homozygous mice.
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mRNA expression analysis
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Strain specific analysis of DPP3 mRNA expression in wild-type C57BL/6 mice and B-hDPP3 mice by RT-PCR. Liver RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hDPP3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human DPP3 primers. Mouse Dpp3 mRNA was detectable only in wild-type mice.Human DPP3 mRNA was detectable only in homozygous B-hDPP3 mice but not in wild-type mice.
