Basic Information
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Targeting strategy
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Gene targeting strategy for B-hEGF mice.
The exons 20-21 of mouse Egf gene were replaced by human EGF exons 20-21(generate the 53-amino acid epidermal growth factor peptide) in B-hEGF mice.
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Protein expression analysis
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Human EGF expression analysis in homozygous B-hEGF mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEGF mice (H/H) and analyzed by ELISA with human EGF ELISA kit. Normal levels of human EGF expression can be detected in homozygous B-hEGF mice. However, lower levels of expression can also detected in WT mice. The ELISA kit may have a cross-reaction with mice.
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mRNA expression analysis
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Strain specific analysis of EGF mRNA expression in wild-type C57BL/6 mice and B-hEGF mice by RT-PCR. Kidney RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEGF mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human EGF primers. Mouse Egf mRNA were detectable only in wild-type C57BL/6 mice. Human EGF mRNA was detectable only in homozygous B-hEGF mice but not in wild-type mice.
