Basic Information
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Targeting strategy
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Gene targeting strategy for B-hEREG mice.
The exons 2~4 of mouse Ereg gene were replaced by human EREG exons 2~4 in B-hEREG mice. -
mRNA expression analysis
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Strain specific analysis of EREG mRNA expression in wild-type C57BL/6 mice and B-hEREG mice by RT-PCR. Small intestine RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEREG mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human EREG primers. Mouse Ereg mRNA was detectable only in wild-type C57BL/6 mice. Human EREG mRNA was detectable only in homozygous B-hEREG mice but not in wild-type mice.
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Protein expression analysis
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Strain specific EREG expression analysis in homozygous B-hEREG mice by ELISA. Colon homogenate were collected from wild-type mice C57BL/6 mice (+/+) and homozygous B-hEREG mice (H/H), and analyzed by ELISA with species-specific EREG ELISA kit. Human EREG was exclusively detectable in homozygous B-hEREG mice but not in wild-type mice (Each mg of total protein contains approximately 150 pg of EREG).
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Summary
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mRNA expression analysis:
Mouse Ereg mRNA was detectable in small intestine of wild-type C57BL/6 mice. Human EREG mRNA was detectable in B-hEREG mice.Protein expression analysis:
Human EREG was exclusively detectable in colon homogenate of homozygous B-hEREG mice but not in wild-type mice .
